| Saturday 19th August 2000 | Premier Division | ||
|---|---|---|---|
| Curzon Ashton | 1-0 | Mossley | |
| Tuesday 22nd August 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 5-4 | Salford City | |
| Saturday 26th August 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-0 | Atherton Collieries | |
| Monday 28th August 2000 | Premier Division | ||
|---|---|---|---|
| Abbey Hey | 1-2 | Mossley | |
| Saturday 2nd September 2000 | F.A. Challenge Cup Preliminary Round | ||
|---|---|---|---|
| Mossley | 4-1 | Hatfield Main | |
| Tuesday 5th September 2000 | Premier Division | ||
|---|---|---|---|
| Cheadle Town | 0-1 | Mossley | |
| Saturday 9th September 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 3-0 | Great Harwood Town | |
| Saturday 16th September 2000 | F.A. Challenge Cup 1st Qualifying Round | ||
|---|---|---|---|
| Mossley | 2-0 | Hebburn Town | |
| Tuesday 19th September 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-0 | Prescot Cables | |
| Saturday 23rd September 2000 | Premier Division | ||
|---|---|---|---|
| Newcastle Town | 0-1 | Mossley | |
| Tuesday 26th September 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 3-0 | Curzon Ashton | |
| Saturday 30th September 2000 | F.A. Challenge Cup 2nd Qualifying Round | ||
|---|---|---|---|
| Altrincham | 0-3 | Mossley | |
| Tuesday 3rd October 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 3-0 | Leek CSOB | |
| Saturday 7th October 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-1 | Woodley Sports | |
| Saturday 14th October 2000 | F.A. Challenge Cup 3rd Qualifying Round | ||
|---|---|---|---|
| Mossley | 1-1 | Frickley Athletic | |
| Tuesday 17th October 2000 | F.A. Challenge Cup 3rd Qualifying Round Replay | ||
|---|---|---|---|
| Frickley Athletic | 3-0 | Mossley | |
| Saturday 21st October 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-3 | Newcastle Town | |
| Tuesday 24th October 2000 | Premier Division | ||
|---|---|---|---|
| Skelmersdale United | 3-2 | Mossley | |
| Saturday 4th November 2000 | Premier Division | ||
|---|---|---|---|
| Kidsgrove Athletic | 1-4 | Mossley | |
| Saturday 11th November 2000 | F.A. Carlsberg Vase 2nd Round | ||
|---|---|---|---|
| Mossley | 2-3 | Consett | |
| (After Extra Time, 2-3 after 90 mins) | |||
| Tuesday 21st November 2000 | Premier Division | ||
|---|---|---|---|
| Salford City | 1-0 | Mossley | |
| Saturday 2nd December 2000 | Premier Division | ||
|---|---|---|---|
| Ramsbottom United | 4-1 | Mossley | |
| Tuesday 12th December 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-2 | St Helens Town | |
| Saturday 23rd December 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-1 | Skelmersdale United | |
| Tuesday 26th December 2000 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-2 | Glossop North End | |
| Saturday 13th January 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-0 | Flixton | |
| Saturday 27th January 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-2 | Ramsbottom United | |
| Saturday 3rd February 2001 | Premier Division | ||
|---|---|---|---|
| Rossendale United | 2-0 | Mossley | |
| Tuesday 13th February 2001 | Premier Division | ||
|---|---|---|---|
| Nantwich Town | 1-5 | Mossley | |
| Saturday 24th February 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 2-1 | Maine Road | |
| Saturday 3rd March 2001 | Premier Division | ||
|---|---|---|---|
| Flixton | 1-6 | Mossley | |
| Tuesday 6th March 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 0-2 | Kidsgrove Athletic | |
| Tuesday 20th March 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 0-1 | Nantwich Town | |
| Saturday 24th March 2001 | Premier Division | ||
|---|---|---|---|
| Atherton Collieries | 0-1 | Mossley | |
| Tuesday 27th March 2001 | Premier Division | ||
|---|---|---|---|
| Woodley Sports | 1-0 | Mossley | |
| Saturday 31st March 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-1 | Clitheroe | |
| Tuesday 3rd April 2001 | Premier Division | ||
|---|---|---|---|
| Prescot Cables | 4-3 | Mossley | |
| Tuesday 10th April 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-2 | Fleetwood Freeport | |
| Saturday 14th April 2001 | Premier Division | ||
|---|---|---|---|
| Leek CSOB | 0-2 | Mossley | |
| Monday 16th April 2001 | Premier Division | ||
|---|---|---|---|
| Glossop North End | 0-4 | Mossley | |
| Wednesday 18th April 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 1-2 | Abbey Hey | |
| Saturday 21st April 2001 | Premier Division | ||
|---|---|---|---|
| Clitheroe | 1-1 | Mossley | |
| Thursday 26th April 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 0-3 | Rossendale United | |
| Saturday 28th April 2001 | Premier Division | ||
|---|---|---|---|
| Maine Road | 0-0 | Mossley | |
| Tuesday 1st May 2001 | Premier Division | ||
|---|---|---|---|
| Fleetwood Freeport | 3-1 | Mossley | |
| Thursday 3rd May 2001 | Premier Division | ||
|---|---|---|---|
| St Helens Town | 1-1 | Mossley | |
| Saturday 5th May 2001 | Premier Division | ||
|---|---|---|---|
| Mossley | 3-2 | Cheadle Town | |
| Monday 7th May 2001 | Premier Division | ||
|---|---|---|---|
| Great Harwood Town | 2-2 | Mossley | |
