| Thursday 13th March 2025 | Premier Division | ||
| 20:00 |  Lower Breck v South Liverpool  | Anfield Sports Community Centre | |
| Friday 14th March 2025 | First Division South | ||
| 20:00 |  Winsford United v Shawbury United  | Barton Stadium | |
| Saturday 15th March 2025 | The Edward Case Cup SF | ||
| 15:00 |  Holker Old Boys v Atherton LR  | The Handmark Engineering Stadium | |
| 15:00 |  Stafford Town v Ashville  | Evans Park | |
| Saturday 15th March 2025 | Premier Division | ||
| 15:00 |  AFC Liverpool v Lower Breck | The Marine Travel Arena | |
| 15:00 |  Barnoldswick Town v Abbey Hey  | Silentnight Stadium | |
| 15:00 |  Burscough v Colne  | The Community Ground | |
| 15:00 |  Cheadle Town v Charnock Richard  | The Ground Up Solutions Stadium | |
| 15:00 |  FC Isle of Man v Pilkington  | The Bowl | |
| 15:00 |  FC St Helens v Bury  | SMS PRO SOCCER PARK | |
| 15:00 |  Irlam v Longridge Town  | The Silver St Stadium | |
| 15:00 |  Ramsbottom United v Litherland REMYCA  | Harry Williams Riverside Stadium | |
| 15:00 | South Liverpool v Glossop North End  | Jericho Lane | |
| 15:00 |  West Didsbury & Chorlton v Padiham  | Step Places Stadium | |
| Saturday 15th March 2025 | First Division North | ||
| 15:00 |  Daisy Hill v AFC Blackpool  | The Plumb Factory Stadium | |
| Saturday 15th March 2025 | First Division South | ||
| 15:00 |  Cammell Laird 1907 v Barnton  | KRCT LTD STADIUM | |
| 15:00 |  Foley Meir v Stockport Georgians  | Trentham Foods Stadium | |
| Sunday 16th March 2025 | Premier Division | ||
| 11:00 | FC Isle of Man v Prestwich Heys  | The Bowl | |
| Monday 17th March 2025 | First Division North | ||
| 19:45 |  Maine Road v Holker Old Boys | Brantingham Road | |
| Tuesday 18th March 2025 | Premier Division | ||
| 19:45 | Colne v Lower Breck | Haffners Stadium | |
| 19:45 | Padiham v Burscough | Ruby Civil Arena | |
| Tuesday 18th March 2025 | First Division North | ||
| 20:00 |  Steeton v Atherton LR | Marley Stadium | |
| Tuesday 18th March 2025 | Lancashire Challenge Trophy SF | ||
| 19:45 | Longridge Town v Southport  | Longridge Town FC | |
| Saturday 22nd March 2025 | Premier Division | ||
| 15:00 | Abbey Hey v Cheadle Town | The Abbey Stadium | |
| 15:00 | Burscough v Litherland REMYCA | The Community Ground | |
| 15:00 | Charnock Richard v Irlam | Mossie Park | |
| 15:00 | Colne v Prestwich Heys | Haffners Stadium | |
| 15:00 | Glossop North End v AFC Liverpool | The Asgard Engineering Stadium | |
| 15:00 | Longridge Town v FC St Helens | Longridge Town FC | |
| 15:00 | Padiham v Squires Gate  | Ruby Civil Arena | |
| 15:00 | Pilkington v Bury | PSD Stadium | |
| 15:00 | Ramsbottom United v West Didsbury & Chorlton | Harry Williams Riverside Stadium | |
| 15:00 |  Stockport Town v Lower Breck | Stockport Sports Village | |
| 16:00 |  Chadderton v Barnoldswick Town | The Falcon Fire Stadium | |
| 18:00 | FC Isle of Man v South Liverpool | The Bowl | |
| Saturday 22nd March 2025 | First Division North | ||
| 15:00 | AFC Blackpool v Daisy Hill | Mechanics Ground | |
| 15:00 |  Ashton Athletic v Droylsden  | Brocstedes Park | |
| 15:00 | Atherton LR v Skelmersdale United  | Crilly Park | |
| 15:00 |  Darwen FC v Nelson  | Multevo Group Anchor Ground | |
| 15:00 |  Euxton Villa v Bacup Borough  | Jim Fowler Memorial Ground | |
| 15:00 |  Garstang v Route One Rovers  | The Riverside | |
| 15:00 | Holker Old Boys v Ashton Town  | The Handmark Engineering Stadium | |
| 15:00 | Steeton v Maine Road | Marley Stadium | |
| 15:00 |  Thornton Cleveleys v Maghull  | Gamble Road | |
| Saturday 22nd March 2025 | First Division South | ||
| 15:00 |  Allscott Heath v Stafford Town | The Giant Stadium | |
| 15:00 |  Alsager Town v Abbey Hulton United  | Wood Park | |
| 15:00 | Cammell Laird 1907 v Stockport Georgians | KRCT LTD STADIUM | |
| 15:00 |  Cheadle Heath Nomads v Runcorn Town  | Ideal Building Systems Stadium | |
| 15:00 | Foley Meir v Eccleshall  | Trentham Foods Stadium | |
| 15:00 |  Market Drayton Town v Winsford United | Greenfields Sports Ground | |
| 15:00 |  Sandbach United v Ashville | Sandbach Community Football Centre | |
| 15:00 |  Wolverhampton Sporting Community v Shawbury United | Pride Park | |
| Wednesday 26th March 2025 | Premier Division | ||
| 19:45 | South Liverpool v Ramsbottom United | Jericho Lane | |
| Friday 28th March 2025 | Premier Division | ||
| 20:00 | AFC Liverpool v Barnoldswick Town | The Marine Travel Arena | |
| Saturday 29th March 2025 | The Macron Cup SF | ||
| 15:00 | AFC Blackpool v Stafford Town | Mechanics Ground | |
| 15:00 | Charnock Richard v Barnton | Mossie Park | |
| Saturday 29th March 2025 | Premier Division | ||
| 15:00 | Abbey Hey v Padiham | The Abbey Stadium | |
| 15:00 | Bury v Squires Gate | Gigg Lane | |
| 15:00 | Cheadle Town v Burscough | The Ground Up Solutions Stadium | |
| 15:00 | Litherland REMYCA v Colne | Litherland Sports Park | |
| 15:00 | Longridge Town v Pilkington | Longridge Town FC | |
| 15:00 | Prestwich Heys v Ramsbottom United | Adie Moran Park | |
| 15:00 | South Liverpool v Irlam | Jericho Lane | |
| 15:00 | Stockport Town v FC St Helens | Stockport Sports Village | |
| 15:00 | West Didsbury & Chorlton v Chadderton | Step Places Stadium | |
| 18:00 | FC Isle of Man v Lower Breck | The Bowl | |
| Saturday 29th March 2025 | First Division North | ||
| 15:00 | Ashton Town v Darwen FC | The Bartons Group Stadium | |
| 15:00 | Bacup Borough v Steeton | Brian Boys West View Stadium | |
| 15:00 | Daisy Hill v Euxton Villa | The Plumb Factory Stadium | |
| 15:00 | Droylsden v Holker Old Boys | The Butchers Arms | |
| 15:00 | Nelson v Atherton LR | The Daisy Arena | |
| 15:00 | Route One Rovers v Maghull | Marley Stadium | |
| 15:00 | Skelmersdale United v Thornton Cleveleys | The Community Ground | |
| Saturday 29th March 2025 | First Division South | ||
| 15:00 | Abbey Hulton United v Wolverhampton Sporting Community | Drayton Beaumont Park | |
| 15:00 | Ashville v Alsager Town | Ray Parker Stadium | |
| 15:00 | Eccleshall v Market Drayton Town | Stoke Signage Stadium | |
| 15:00 |  New Mills v Cammell Laird 1907 | Church Lane | |
| 15:00 | Runcorn Town v Foley Meir | Viridor Community Stadium | |
| 15:00 | Shawbury United v Sandbach United | Shawbury Utd Community Sports and Recreational Centre | |
| 15:00 | Stockport Georgians v Winsford United | Cromley Road | |
| Tuesday 1st April 2025 | First Division North | ||
| 19:45 | Atherton LR v Droylsden | Crilly Park | |
| 19:45 | Euxton Villa v Route One Rovers | Jim Fowler Memorial Ground | |
| 19:45 | Holker Old Boys v Garstang | The Handmark Engineering Stadium | |
| 19:45 | Maghull v Ashton Town | DT Hughes Community Stadium | |
| 19:45 | Skelmersdale United v Daisy Hill | The Community Ground | |
| 19:45 | Thornton Cleveleys v AFC Blackpool | Gamble Road | |
| Tuesday 1st April 2025 | First Division South | ||
| 19:45 | Market Drayton Town v Sandbach United | Greenfields Sports Ground | |
| 19:45 | Runcorn Town v Ashville | Viridor Community Stadium | |
| 19:45 | Shawbury United v Allscott Heath | Shawbury Utd Community Sports and Recreational Centre | |
| 19:45 | Stafford Town v Abbey Hulton United | Evans Park | |
| 19:45 | Stockport Georgians v New Mills | Cromley Road | |
| 19:45 | Winsford United v Cheadle Heath Nomads | Barton Stadium | |
| Wednesday 2nd April 2025 | First Division North | ||
| 19:45 | Darwen FC v Ashton Athletic | Multevo Group Anchor Ground | |
| 20:00 | Bacup Borough v Maine Road | Brian Boys West View Stadium | |
| Wednesday 2nd April 2025 | First Division South | ||
| 19:45 | Eccleshall v Wolverhampton Sporting Community | Stoke Signage Stadium | |
| 19:45 | Foley Meir v Alsager Town | Trentham Foods Stadium | |
| Thursday 13th March 2025 Premier Division |
| Lower Breck v South Liverpool (20:00) |
| Friday 14th March 2025 First Division South |
| Winsford United v Shawbury United (20:00) |
| Saturday 15th March 2025 The Edward Case Cup Semi Finals |
| Holker Old Boys v Atherton LR (15:00) |
| Stafford Town v Ashville (15:00) |
| Saturday 15th March 2025 Premier Division |
| AFC Liverpool v Lower Breck (15:00) |
| Barnoldswick Town v Abbey Hey (15:00) |
| Burscough v Colne (15:00) |
| Cheadle Town v Charnock Richard (15:00) |
| FC Isle of Man v Pilkington (15:00) |
| FC St Helens v Bury (15:00) |
| Irlam v Longridge Town (15:00) |
| Ramsbottom United v Litherland REMYCA (15:00) |
| South Liverpool v Glossop North End (15:00) |
| West Didsbury & Chorlton v Padiham (15:00) |
| Saturday 15th March 2025 First Division North |
| Daisy Hill v AFC Blackpool (15:00) |
| Saturday 15th March 2025 First Division South |
| Cammell Laird 1907 v Barnton (15:00) |
| Foley Meir v Stockport Georgians (15:00) |
| Sunday 16th March 2025 Premier Division |
| FC Isle of Man v Prestwich Heys (11:00) |
| Monday 17th March 2025 First Division North |
| Maine Road v Holker Old Boys (19:45) |
| Tuesday 18th March 2025 Premier Division |
| Colne v Lower Breck (19:45) |
| Padiham v Burscough (19:45) |
| Tuesday 18th March 2025 First Division North |
| Steeton v Atherton LR (20:00) |
| Tuesday 18th March 2025 Lancashire Challenge Trophy Semi Finals |
| Longridge Town v Southport (19:45) |
| Saturday 22nd March 2025 Premier Division |
| Abbey Hey v Cheadle Town (15:00) |
| Burscough v Litherland REMYCA (15:00) |
| Charnock Richard v Irlam (15:00) |
| Colne v Prestwich Heys (15:00) |
| Glossop North End v AFC Liverpool (15:00) |
| Longridge Town v FC St Helens (15:00) |
| Padiham v Squires Gate (15:00) |
| Pilkington v Bury (15:00) |
| Ramsbottom United v West Didsbury & Chorlton (15:00) |
| Stockport Town v Lower Breck (15:00) |
| Chadderton v Barnoldswick Town (16:00) |
| FC Isle of Man v South Liverpool (18:00) |
| Saturday 22nd March 2025 First Division North |
| AFC Blackpool v Daisy Hill (15:00) |
| Ashton Athletic v Droylsden (15:00) |
| Atherton LR v Skelmersdale United (15:00) |
| Darwen FC v Nelson (15:00) |
| Euxton Villa v Bacup Borough (15:00) |
| Garstang v Route One Rovers (15:00) |
| Holker Old Boys v Ashton Town (15:00) |
| Steeton v Maine Road (15:00) |
| Thornton Cleveleys v Maghull (15:00) |
| Saturday 22nd March 2025 First Division South |
| Allscott Heath v Stafford Town (15:00) |
| Alsager Town v Abbey Hulton United (15:00) |
| Cammell Laird 1907 v Stockport Georgians (15:00) |
| Cheadle Heath Nomads v Runcorn Town (15:00) |
| Foley Meir v Eccleshall (15:00) |
| Market Drayton Town v Winsford United (15:00) |
| Sandbach United v Ashville (15:00) |
| Wolverhampton Sporting Community v Shawbury United (15:00) |
| Wednesday 26th March 2025 Premier Division |
| South Liverpool v Ramsbottom United (19:45) |
| Friday 28th March 2025 Premier Division |
| AFC Liverpool v Barnoldswick Town (20:00) |
| Saturday 29th March 2025 The Macron Cup Semi Finals |
| AFC Blackpool v Stafford Town (15:00) |
| Charnock Richard v Barnton (15:00) |
| Saturday 29th March 2025 Premier Division |
| Abbey Hey v Padiham (15:00) |
| Bury v Squires Gate (15:00) |
| Cheadle Town v Burscough (15:00) |
| Litherland REMYCA v Colne (15:00) |
| Longridge Town v Pilkington (15:00) |
| Prestwich Heys v Ramsbottom United (15:00) |
| South Liverpool v Irlam (15:00) |
| Stockport Town v FC St Helens (15:00) |
| West Didsbury & Chorlton v Chadderton (15:00) |
| FC Isle of Man v Lower Breck (18:00) |
| Saturday 29th March 2025 First Division North |
| Ashton Town v Darwen FC (15:00) |
| Bacup Borough v Steeton (15:00) |
| Daisy Hill v Euxton Villa (15:00) |
| Droylsden v Holker Old Boys (15:00) |
| Nelson v Atherton LR (15:00) |
| Route One Rovers v Maghull (15:00) |
| Skelmersdale United v Thornton Cleveleys (15:00) |
| Saturday 29th March 2025 First Division South |
| Abbey Hulton United v Wolverhampton Sporting Community (15:00) |
| Ashville v Alsager Town (15:00) |
| Eccleshall v Market Drayton Town (15:00) |
| New Mills v Cammell Laird 1907 (15:00) |
| Runcorn Town v Foley Meir (15:00) |
| Shawbury United v Sandbach United (15:00) |
| Stockport Georgians v Winsford United (15:00) |
| Tuesday 1st April 2025 First Division North |
| Atherton LR v Droylsden (19:45) |
| Euxton Villa v Route One Rovers (19:45) |
| Holker Old Boys v Garstang (19:45) |
| Maghull v Ashton Town (19:45) |
| Skelmersdale United v Daisy Hill (19:45) |
| Thornton Cleveleys v AFC Blackpool (19:45) |
| Tuesday 1st April 2025 First Division South |
| Market Drayton Town v Sandbach United (19:45) |
| Runcorn Town v Ashville (19:45) |
| Shawbury United v Allscott Heath (19:45) |
| Stafford Town v Abbey Hulton United (19:45) |
| Stockport Georgians v New Mills (19:45) |
| Winsford United v Cheadle Heath Nomads (19:45) |
| Wednesday 2nd April 2025 First Division North |
| Darwen FC v Ashton Athletic (19:45) |
| Bacup Borough v Maine Road (20:00) |
| Wednesday 2nd April 2025 First Division South |
| Eccleshall v Wolverhampton Sporting Community (19:45) |
| Foley Meir v Alsager Town (19:45) |
