| Sat 26/07/25 | Darwen FC v Thornton Cleveleys | 0-0 | |  | |  | | |
| Sat 02/08/25 | Darwen FC v MSB Woolton | 2-1 | | | | | | |
| Sat 09/08/25 | Squires Gate v Darwen FC | 2-1 | | | | | | |
| Sat 16/08/25 | Holker Old Boys v Darwen FC | 1-2 | | | | | | |
| Sat 30/08/25 | Darwen FC v AFC Blackpool | 0-2 | | | | | | |
| Sat 06/09/25 | Darwen FC v Fulwood Amateurs | 1-1 | | | | | | |
| Sat 13/09/25 | Steeton v Darwen FC | 1-2 | | | | | | |
| Sat 20/09/25 | Armthorpe Welfare v Darwen FC (CUP) | 2-6 | | | | | | |
| Sat 27/09/25 | Darwen FC v Garstang (CUP) | 0-0 | | | | | | |
| Sat 04/10/25 | Darwen FC v Maghull | 5-2 | | | | | | |
| Sat 11/10/25 | Daisy Hill v Darwen FC | 3-2 | | | | | | |
| Sat 17/01/26 | Ashton Town v Darwen FC | 2-0 | | | | | |  |
| Tue 20/01/26 | Fulwood Amateurs v Darwen FC | 2-2 | | | | | | |
| Sat 24/01/26 | Darwen FC v Maine Road | 2-1 | | | | | | |
| Fri 30/01/26 | Barnton v Darwen FC (CUP) | 2-2 | | | | | | |
| Wed 04/02/26 | Bacup Borough v Darwen FC | 1-1 | | | | | | |
| Sat 07/02/26 | Thornton Cleveleys v Darwen FC | 3-7 | | | | | | |
| Sat 14/02/26 | Darwen FC v Daisy Hill | 3-2 | | | | | | |
| Sat 21/02/26 | Maine Road v Darwen FC | 0-1 | | | | | | |
| Fri 27/02/26 | Darwen FC v Colne | 0-0 | | | | | | |
| Sat 07/03/26 | Garstang v Darwen FC | 2-1 | | | | | | |
| Sat 14/03/26 | Darwen FC v Stockport Georgians (CUP) | 1-0 | | | | | | |
| Sat 21/03/26 | AFC Blackpool v Darwen FC | 1-2 | | | | | | |
| Wed 25/03/26 | Darwen FC v Steeton | 2-0 | | | | | | |
| Sat 28/03/26 | MSB Woolton v Darwen FC | 2-1 | | | | | | |
| Sat 04/04/26 | Darwen FC v Squires Gate | 2-1 | | | | | | |
| Mon 06/04/26 | Nelson v Darwen FC | 1-0 | | | | | | |
| Sat 18/04/26 | Droylsden v Darwen FC | 2-2 | | | | | | |
| Thu 30/04/26 | Darwen FC v Runcorn Town (CUP) | 2-3 | | | | | | |
