| Sat 15/11/25 | Ashton Town v Darwen FC (CUP) | 2-3 |  | | | | | |
| Wed 19/11/25 | Darwen FC v Bacup Borough | 1-1 | | | | | | |
| Sat 22/11/25 | Maghull v Darwen FC | 1-1 | | | | | | |
| Sat 29/11/25 | Darwen FC v Ashton Town | 1-0 | |  | | | | |
| Sat 06/12/25 | Ashton Athletic v Darwen FC | 2-2 | | | | | | |
| Sat 13/12/25 | Darwen FC v Holker Old Boys | 3-3 | | | | | | |
| Fri 26/12/25 | Darwen FC v Nelson | 0-2 | | | |  | | |
| Sat 10/01/26 | Darwen FC v Droylsden * | 2-3 | | | | | | |
| Sat 17/01/26 | Ashton Town v Darwen FC | 2-0 | | | | | | |
| Tue 20/01/26 | Fulwood Amateurs v Darwen FC | 2-2 | | | | | | |
| Sat 24/01/26 | Darwen FC v Maine Road | 2-1 | | | | | | |
| Fri 30/01/26 | Barnton v Darwen FC (CUP) | 2-2 | | | | | | |
| Wed 04/02/26 | Bacup Borough v Darwen FC * | 1-1 | | | | | | |
| Sat 07/02/26 | Thornton Cleveleys v Darwen FC | 3-7 | | | | | | |
| Sat 14/02/26 | Darwen FC v Daisy Hill | 3-2 | | | | | | |
| Sat 21/02/26 | Maine Road v Darwen FC | 0-1 | | | | | | |
| Fri 27/02/26 | Darwen FC v Colne | 0-0 | | | | | | |
| Sat 07/03/26 | Garstang v Darwen FC * | 2-1 | | | | | | |
| Sat 14/03/26 | Darwen FC v Stockport Georgians (CUP) | 1-0 | | | | | | |
| Sat 21/03/26 | AFC Blackpool v Darwen FC | 1-2 | | | | | | |
| Wed 25/03/26 | Darwen FC v Steeton | 2-0 | | | | | | |
| Sat 28/03/26 | MSB Woolton v Darwen FC | 2-1 | | | | | | |
| Sat 04/04/26 | Darwen FC v Squires Gate | 2-1 | | | | | | |
| Mon 06/04/26 | Nelson v Darwen FC | 1-0 | | | | | | |
