| Tue 08/08/23 | Ashton Athletic v Darwen FC | 2-1 | | | | | | |
| Sat 12/08/23 | Darwen FC v South Liverpool | 1-2 | | | | | | |
| Tue 15/08/23 | FC St Helens v Darwen FC * | 2-1 | | | | | | |
| Sat 19/08/23 | AFC Blackpool v Darwen FC | 0-3 | |  | | | | |
| Wed 23/08/23 | Darwen FC v Holker Old Boys | 1-2 | | | |  | | |
| Sat 26/08/23 | Darwen FC v Shelley | 3-3 | | | | | | |
| Mon 28/08/23 | Ashton Town v Darwen FC * | 6-2 | | | | | | |
| Sat 02/09/23 | Darwen FC v Nelson | 3-0 | | | | | | |
| Sat 09/09/23 | Darwen FC v Runcorn Town | 4-5 |  | | | | | |
| Sat 16/09/23 | Atherton LR v Darwen FC * | 2-1 | | | | | | |
| Sat 23/09/23 | Darwen FC v Garstang * | 2-2 | | | | | | |
| Sat 30/09/23 | Shelley v Darwen FC | 0-1 | | | | | | |
| Sat 07/10/23 | Darwen FC v AFC Blackpool | 6-1 | | | | | | |
| Wed 11/10/23 | Darwen FC v FC St Helens (CUP) | 4-1 | | | | | | |
| Sat 14/10/23 | Darwen FC v Euxton Villa | 3-2 | | | | | | |
| Wed 18/10/23 | Darwen FC v Padiham (CUP) * | 0-0 | | | | | | |
| Tue 24/10/23 | Heaton Stannington v Darwen FC (CUP) | 3-0 | | | | | | |
| Sat 28/10/23 | Runcorn Town v Darwen FC | 2-3 | | | | | | |
| Sat 04/11/23 | Darwen FC v Steeton | 3-1 | | | | | | |
| Sat 11/11/23 | Darwen FC v Euxton Villa (CUP) | 1-3 | | | | | | |
| Sat 18/11/23 | Droylsden v Darwen FC (CUP) * | 2-1 | | | | | | |
| Wed 22/11/23 | Darwen FC v Ashton Town | 1-4 | | | | | | |
| Sat 09/12/23 | Route One Rovers v Darwen FC | 3-1 | | | | | | |
| Sat 16/12/23 | Darwen FC v Ashton Athletic | 2-2 | | | | | | |
| Tue 26/12/23 | Darwen FC v Bacup Borough * | 2-1 | | | | | | |
| Sat 30/12/23 | South Liverpool v Darwen FC * | 3-3 | | | | | | |
| Sat 06/01/24 | Darwen FC v Atherton LR * | 2-1 | | | | | | |
| Mon 08/01/24 | Daisy Hill v Darwen FC | 0-1 | | | | | | |
| Sat 10/02/24 | Holker Old Boys v Darwen FC | 1-2 | | | | | | |
| Sat 17/02/24 | Darwen FC v Daisy Hill | 0-0 | | | | | | |
| Sun 03/03/24 | Euxton Villa v Darwen FC * | 3-0 | | | | | | |
| Tue 12/03/24 | Nelson v Darwen FC | 1-1 | | | | | | |
| Sat 23/03/24 | Darwen FC v Route One Rovers * | 3-3 | | | | | | |
| Mon 01/04/24 | Bacup Borough v Darwen FC | 2-5 | | | | | | |
| Sat 06/04/24 | Ilkley Town v Darwen FC | 5-3 | | | | | | |
| Sat 13/04/24 | Darwen FC v FC St Helens | 4-3 | | | | | | |
