| Sat 26/07/25 | Euxton Villa v Prestwich Heys | 1-1 | | | | | | |
| Tue 29/07/25 | Prestwich Heys v Padiham | 0-2 | | | | | | |
| Sat 02/08/25 | Wythenshawe Town v Prestwich Heys (CUP) | 2-4 | | | | | | |
| Fri 08/08/25 | Prestwich Heys v Burscough | 0-2 | | | | | | |
| Mon 11/08/25 | Stockport Town v Prestwich Heys * | 1-1 | | | | | | |
| Fri 15/08/25 | Atherton Collieries v Prestwich Heys (CUP) | 2-0 | | | | | | |
| Sat 23/08/25 | 1874 Northwich v Prestwich Heys (CUP) | 0-2 | |  | | | | |
| Mon 25/08/25 | Barnoldswick Town v Prestwich Heys | 2-2 | | | | | | |
| Sat 30/08/25 | Prestwich Heys v Cheadle Town * | 1-1 | | | | | | |
| Sat 06/09/25 | Irlam v Prestwich Heys | 2-1 | | | |  | | |
| Tue 09/09/25 | Prestwich Heys v West Didsbury & Chorlton | 2-1 | | | | | | |
| Sat 13/09/25 | Prestwich Heys v South Liverpool | 3-0 | | | | | | |
| Tue 16/09/25 | Glossop North End v Prestwich Heys | 1-2 | | | | | | |
| Tue 23/09/25 | Prestwich Heys v Charnock Richard | 3-3 | | | | | | |
| Sat 27/09/25 | Stockport Town v Prestwich Heys (CUP) | 2-3 | | | | | | |
| Tue 07/10/25 | FC St Helens v Prestwich Heys | 0-2 | | | | | | |
| Sat 11/10/25 | Ramsbottom United v Prestwich Heys (CUP) | 3-2 | | | | | | |
| Tue 14/10/25 | Prestwich Heys v Pilkington | 2-0 | | | | | | |
| Sat 18/10/25 | Prestwich Heys v Maltby Main (CUP) | 9-1 | | | | | | |
| Tue 21/10/25 | Abbey Hey v Prestwich Heys | 2-1 | | | | | | |
| Sat 25/10/25 | Padiham v Prestwich Heys | 0-2 | | | | | | |
| Tue 28/10/25 | Ramsbottom United v Prestwich Heys | 3-0 | | | | | | |
| Sat 01/11/25 | Prestwich Heys v Ramsbottom United | 0-3 | | | | | | |
| Sat 22/11/25 | Prestwich Heys v Glossop North End | 0-2 | | | | | | |
| Wed 26/11/25 | Litherland REMYCA v Prestwich Heys | 0-2 | | | | | | |
| Sat 29/11/25 | Prestwich Heys v FC Isle of Man | 5-0 | | | | | | |
