| Sat 09/09/23 | FC Isle of Man v Prestwich Heys * | 0-2 | |  | | | | |
| Tue 12/09/23 | Prestwich Heys v Ramsbottom United | 3-0 | | | | | | |
| Sat 16/09/23 | Chadderton v Prestwich Heys | 2-3 | | | | | | |
| Sat 23/09/23 | Prestwich Heys v Parkgate (CUP) | 1-2 | | | | | | |
| Sat 30/09/23 | Lower Breck v Prestwich Heys | 2-5 | | | | | | |
| Tue 03/10/23 | Barnoldswick Town v Prestwich Heys | 3-3 | | | | | | |
| Sat 07/10/23 | Prestwich Heys v Skelmersdale United * | 2-1 | | | | | | |
| Tue 10/10/23 | Prestwich Heys v Kendal Town | 2-2 | | | | | | |
| Sat 16/12/23 | Prestwich Heys v Lower Breck * | 2-1 | | | | | | |
| Tue 26/12/23 | Bury v Prestwich Heys * | 2-0 | | | |  | | |
| Sat 06/01/24 | Wythenshawe v Prestwich Heys | 0-1 | | | | | | |
| Sat 27/01/24 | Prestwich Heys v Barnoldswick Town | 3-1 | | | | | | |
| Tue 30/01/24 | Prestwich Heys v Glossop North End | 3-2 |  | | | | | |
| Sat 03/02/24 | Padiham v Prestwich Heys | 1-0 | | |  | | | |
| Sat 10/02/24 | Prestwich Heys v FC Isle of Man * | 3-4 | | | | | | |
| Sat 17/02/24 | Alsager Town v Prestwich Heys (CUP) * | 0-0 | | | | | | |
| Sat 24/02/24 | Burscough v Prestwich Heys * | 1-1 | | | | | | |
| Tue 27/02/24 | West Didsbury & Chorlton v Prestwich Heys | 1-1 | | | | | | |
| Tue 05/03/24 | Kendal Town v Prestwich Heys | 1-0 | | | | | | |
| Tue 19/03/24 | Prestwich Heys v Longridge Town * | 3-1 | | | | | | |
| Sat 23/03/24 | Skelmersdale United v Prestwich Heys * | 1-0 | | | | | | |
| Sat 30/03/24 | Prestwich Heys v Colne | 1-0 | | | | | | |
| Mon 01/04/24 | Prestwich Heys v Bury | 1-2 | | | | | | |
| Thu 04/04/24 | Prestwich Heys v Chadderton * | 5-2 | | | | | | |
| Sat 06/04/24 | Irlam v Prestwich Heys * | 2-1 | | | | | | |
| Sat 20/04/24 | Pilkington v Prestwich Heys | 2-3 | | | | | | |
