| Date | Match |
|---|---|
| 31/07/24 | Garstang 1-3 Holker OB |
| 03/08/24 | Holker OB 4-0 Droylsden   |
| 06/08/24 | Holker OB 2-2 Euxton Villa |
| 10/08/24 | Ashton Town 0-3 Holker OB |
| 12/08/24 | Route One Rovers 1-3 Holker OB |
| 17/08/24 | Holker OB 4-1 Nelson |
| 21/08/24 | AFC Blackpool 5-1 Holker OB |
| 31/08/24 | Holker OB 3-1 Bacup Borough  |
| 07/09/24 | Darwen FC 2-4 Holker OB |
| 21/09/24 | Crook Town 0-0 Holker OB |
| 05/10/24 | Skelmersdale Utd 0-9 Holker OB |
| 08/10/24 | Holker OB 1-0 Darwen FC * |
| 26/10/24 | Holker OB 2-4 AFC Blackpool |
| 02/11/24 | Atherton LR 2-0 Holker OB |
| 09/11/24 | Daisy Hill 1-2 Holker OB |
| 16/11/24 | Bacup Borough 1-1 Holker OB |
| 30/11/24 | Holker OB 0-2 Route One Rovers |
| 26/12/24 | Thornton Cleveleys 1-3 Holker OB |
| 04/01/25 | Euxton Villa 3-2 Holker OB |
| 01/02/25 | Abbey Hulton Utd 1-3 Holker OB * |
| 08/02/25 | Holker OB 1-1 Darwen FC * |
| 15/02/25 | Holker OB 2-0 Steeton * |
| 22/02/25 | Ashton Athletic 1-0 Holker OB |
| 01/03/25 | Maghull 1-1 Holker OB |
| 08/03/25 | Holker OB 3-1 Atherton LR |
| 15/03/25 | Holker OB 1-2 Atherton LR |
| 17/03/25 | Maine Road 0-0 Holker OB |
* Indicates appearance as a substitute
| Comp | Team | App |
|---|---|---|
| The Isuzu FA Vase | Holker Old Boys | 1 |
| The Edward Case Cup | Holker Old Boys | 4 |
| First Division North | Holker Old Boys | 22 |
| Comp | YC | SB | RC |
|---|---|---|---|
| First Division North | 7 | 0 | 1 |
| The Edward Case Cup | 2 | 0 | 0 |
