| Sat 14/08/21 | Barnoldswick Town v Prestwich Heys | 3-1 | | | | |  |
| Wed 18/08/21 | Longridge Town v Barnoldswick Town * | 2-5 | | | | | |
| Sat 21/08/21 | Barnoldswick Town v Ramsbottom United * | 5-4 | | | | | |
| Tue 07/09/21 | Burscough v Barnoldswick Town | 2-0 | | | | | |
| Sat 11/09/21 | Barnoldswick Town v AFC Blackpool | 3-3 | | | | | |
| Tue 28/09/21 | Charnock Richard v Barnoldswick Town * | 4-1 | | | | | |
| Sat 09/10/21 | Barnoldswick Town v Congleton Town | 2-2 |  | |  | | |
| Wed 13/10/21 | Barnoldswick Town v Litherland REMYCA | 4-1 | | | | | |
| Sat 16/10/21 | Lower Breck v Barnoldswick Town | 2-1 | | | | | |
| Tue 26/10/21 | Wythenshawe Town v Barnoldswick Town | 1-2 | | | | | |
| Sat 30/10/21 | Barnoldswick Town v Skelmersdale United | 2-2 | | | | | |
| Sat 06/11/21 | AFC Liverpool v Barnoldswick Town | 1-3 | | | | | |
| Wed 10/11/21 | Barnoldswick Town v Northwich Victoria | 1-4 | | | | | |
| Sat 13/11/21 | Barnoldswick Town v Ashton Athletic | 2-2 | | | | | |
| Tue 16/11/21 | Liversedge v Barnoldswick Town | 3-0 | | | | | |
| Sat 27/11/21 | Barnoldswick Town v Burscough * | 1-1 | | | | | |
| Sat 04/12/21 | Macclesfield v Barnoldswick Town * | 2-0 | | | | | |
| Wed 19/01/22 | Litherland REMYCA v Barnoldswick Town | 4-0 | | | | | |
| Sat 22/01/22 | Barnoldswick Town v AFC Liverpool | 2-2 | | | | | |
| Tue 08/02/22 | Congleton Town v Barnoldswick Town | 2-2 | | | | | |
| Sat 12/02/22 | Barnoldswick Town v Runcorn Town | 1-2 | | | | | |
| Sat 19/02/22 | Skelmersdale United v Barnoldswick Town | 3-0 | | | | | |
| Sat 26/02/22 | Vauxhall Motors v Barnoldswick Town | 2-1 | | | | | |
| Sat 12/03/22 | Barnoldswick Town v Padiham | 2-0 | | | | | |
| Tue 15/03/22 | Prestwich Heys v Barnoldswick Town | 0-2 | | | | | |
| Sat 19/03/22 | Barnoldswick Town v Lower Breck | 1-0 | | | | | |
| Wed 23/03/22 | Barnoldswick Town v Wythenshawe Town | 1-1 | | | | | |
| Sat 26/03/22 | Ashton Athletic v Barnoldswick Town | 2-2 | | | | | |
| Wed 06/04/22 | Barnoldswick Town v Steeton | 3-2 | | | | | |
| Tue 12/04/22 | Bury AFC v Barnoldswick Town | 3-1 | | | | | |
