| Sat 09/08/25 | FC Isle of Man v Glossop North End | 2-3 | | | | | | |
| Tue 12/08/25 | Glossop North End v Wythenshawe | 2-2 | | | | | |  |
| Sat 23/08/25 | Glossop North End v Bacup Borough (CUP) | 1-1 | | | | | | |
| Mon 25/08/25 | Abbey Hey v Glossop North End | 2-0 | | | | | | |
| Sat 30/08/25 | Glossop North End v South Liverpool | 0-1 | | | | | | |
| Sat 06/09/25 | Glossop North End v Pilkington | 0-4 | | | | | | |
| Tue 09/09/25 | Padiham v Glossop North End | 3-1 | | | |  | | |
| Sat 13/09/25 | Litherland REMYCA v Glossop North End | 1-2 | | | | | | |
| Tue 16/09/25 | Glossop North End v Prestwich Heys | 1-2 | | | | | | |
| Sat 20/09/25 | West Didsbury & Chorlton v Glossop North End | 1-2 | | | | | | |
| Tue 23/09/25 | Cheadle Town v Glossop North End | 0-1 | |  | | | | |
| Sat 27/09/25 | Cheadle Town v Glossop North End (CUP) | 3-2 | | | | | | |
| Tue 30/09/25 | Glossop North End v Atherton LR | 0-2 | | | | | | |
| Sat 04/10/25 | Glossop North End v Chadderton | 0-3 | | | | | | |
| Tue 07/10/25 | Glossop North End v Stockport Town | 1-2 | | | | | | |
| Sat 11/10/25 | Pilkington v Glossop North End | 2-0 | | | | | | |
| Sat 18/10/25 | Longridge Town v Glossop North End | 2-1 | | | | | | |
| Tue 21/10/25 | Ramsbottom United v Glossop North End | 1-0 | | | | | | |
| Sat 25/10/25 | Glossop North End v Charnock Richard | 2-0 | | | | | | |
| Sat 01/11/25 | Atherton LR v Glossop North End | 2-3 | | | | | | |
| Tue 04/11/25 | Shirebrook Town v Glossop North End (CUP) | 0-5 |  | | | | | |
| Sat 08/11/25 | Stockport Town v Glossop North End | 1-2 | | | | | | |
| Sat 15/11/25 | Burscough v Glossop North End | 1-2 | | | | | | |
| Tue 18/11/25 | Glossop North End v FC St Helens | 1-3 | | | | | | |
| Sat 22/11/25 | Prestwich Heys v Glossop North End | 0-2 | | | | | | |
| Tue 25/11/25 | Wythenshawe Town v Glossop North End (CUP) * | 5-3 | | | | | | |
| Sat 29/11/25 | Euxton Villa v Glossop North End | 3-2 | | | | | | |
| Tue 02/12/25 | Glossop North End v Matlock Town (CUP) | 2-1 | | | | | | |
| Sat 13/12/25 | Chadderton v Glossop North End | 3-6 | | | | | | |
| Tue 16/12/25 | Glossop North End v AFC Liverpool | 1-1 | | | | | | |
| Sat 20/12/25 | Glossop North End v FC Isle of Man | 2-3 | | | | | | |
| Fri 26/12/25 | Irlam v Glossop North End | 0-1 | | | | | | |
