| Sat 03/08/24 | Maine Road v Darwen FC * | 2-0 | | | | | | |
| Wed 07/08/24 | Darwen FC v Thornton Cleveleys | 3-4 | | | | | | |
| Sat 10/08/24 | Darwen FC v AFC Blackpool | 2-1 | | | | | | |
| Tue 13/08/24 | Ashton Athletic v Darwen FC | 1-5 | | | | | | |
| Sat 17/08/24 | Darwen FC v Atherton LR | 1-2 |  | | | | | |
| Wed 21/08/24 | Nelson v Darwen FC | 3-0 | | | | | | |
| Sat 24/08/24 | Dearne & District v Darwen FC (CUP) | 0-1 | |  | | | | |
| Sat 31/08/24 | Darwen FC v Garstang | 1-0 | | | | | | |
| Sat 07/09/24 | Darwen FC v Holker Old Boys | 2-4 | | | | | | |
| Sat 28/09/24 | Route One Rovers v Darwen FC * | 3-2 | | | | | | |
| Sat 05/10/24 | Darwen FC v Steeton | 3-2 | | | | | | |
| Tue 08/10/24 | Holker Old Boys v Darwen FC (CUP) | 1-0 | | | | | | |
| Sat 12/10/24 | AFC Blackpool v Darwen FC | 3-2 | | | | | | |
| Sat 19/10/24 | Darwen FC v Harrogate Railway Athletic (CUP) * | 3-0 | | | | | | |
| Sat 26/10/24 | Darwen FC v Ashton Town * | 3-0 | | | | | | |
| Sat 02/11/24 | Skelmersdale United v Darwen FC * | 1-6 | | | | | | |
| Sat 09/11/24 | Winsford United v Darwen FC (CUP) | 6-1 | | |  | | | |
| Sat 16/11/24 | Darwen FC v Daisy Hill * | 4-1 | | | | | | |
| Sat 30/11/24 | Atherton LR v Darwen FC * | 0-2 | | | | | | |
| Sat 14/12/24 | Garstang v Darwen FC | 0-2 | | | | | | |
| Thu 26/12/24 | Darwen FC v Euxton Villa | 4-1 | | | | | | |
| Sat 28/12/24 | Darwen FC v Bacup Borough | 1-0 | | | | | | |
| Sat 11/01/25 | Darwen FC v Maghull | 1-3 | | | |  | |  |
| Sat 18/01/25 | Darwen FC v Route One Rovers | 4-0 | | | | | | |
| Sat 01/02/25 | Darwen FC v Droylsden | 0-1 | | | | | | |
| Sat 15/02/25 | Thornton Cleveleys v Darwen FC | 1-2 | | | | | | |
| Sun 02/03/25 | Droylsden v Darwen FC | 3-2 | | | | | | |
| Sat 29/03/25 | Ashton Town v Darwen FC * | 0-2 | | | | | | |
| Wed 02/04/25 | Darwen FC v Ashton Athletic | 4-1 | | | | | | |
| Sat 05/04/25 | Steeton v Darwen FC | 0-3 | | | | | | |
| Sat 12/04/25 | Darwen FC v Maine Road | 2-1 | | | | | | |
