| Sat 31/07/21 | Runcorn Town v Barnoldswick Town | 1-2 | | | | | |
| Tue 03/08/21 | Barnoldswick Town v Squires Gate | 2-2 | | |  | | |
| Sat 07/08/21 | Barnoldswick Town v Runcorn Town | 6-1 | | | | | |
| Sat 14/08/21 | Barnoldswick Town v Prestwich Heys | 3-1 | | | | | |
| Sat 28/08/21 | Barnoldswick Town v Macclesfield | 1-2 | | | | | |
| Mon 30/08/21 | Padiham v Barnoldswick Town | 0-0 | | | | | |
| Sat 04/09/21 | Barnoldswick Town v Pontefract Collieries | 0-3 | | | | | |
| Tue 07/09/21 | Burscough v Barnoldswick Town | 2-0 | | | | | |
| Sat 11/09/21 | Barnoldswick Town v AFC Blackpool * | 3-3 | | | | | |
| Sat 18/09/21 | Barnoldswick Town v Vauxhall Motors | 2-3 | | | | | |
| Tue 21/09/21 | Irlam v Barnoldswick Town | 0-3 | | | | | |
| Sat 25/09/21 | Barnoldswick Town v Heaton Stannington | 1-2 | | | | | |
| Tue 28/09/21 | Charnock Richard v Barnoldswick Town | 4-1 | | | | | |
| Sat 02/10/21 | Winsford United v Barnoldswick Town | 1-2 | | | | | |
| Sat 09/10/21 | Barnoldswick Town v Congleton Town | 2-2 | | | | | |
| Wed 13/10/21 | Barnoldswick Town v Litherland REMYCA | 4-1 | | | | | |
| Sat 16/10/21 | Lower Breck v Barnoldswick Town | 2-1 | | | | | |
| Tue 26/10/21 | Wythenshawe Town v Barnoldswick Town * | 1-2 | | | | | |
| Sat 30/10/21 | Barnoldswick Town v Skelmersdale United | 2-2 | | | | | |
| Sat 06/11/21 | AFC Liverpool v Barnoldswick Town | 1-3 | | | | | |
| Wed 10/11/21 | Barnoldswick Town v Northwich Victoria | 1-4 | | | | | |
| Sat 13/11/21 | Barnoldswick Town v Ashton Athletic | 2-2 | | | | | |
| Tue 16/11/21 | Liversedge v Barnoldswick Town | 3-0 | | | | | |
| Sat 20/11/21 | Squires Gate v Barnoldswick Town | 4-2 | | | | | |
| Sat 27/11/21 | Barnoldswick Town v Burscough | 1-1 | |  | | | |
| Sat 04/12/21 | Macclesfield v Barnoldswick Town | 2-0 | | | | | |
| Sat 18/12/21 | Barnoldswick Town v Winsford United | 2-2 | | | | | |
| Mon 27/12/21 | Barnoldswick Town v Longridge Town | 2-2 | | | | |  |
| Sat 15/01/22 | Barnoldswick Town v Irlam | 2-2 | | | | | |
| Wed 19/01/22 | Litherland REMYCA v Barnoldswick Town * | 4-0 | | | | | |
| Sat 22/01/22 | Barnoldswick Town v AFC Liverpool | 2-2 | | | | | |
| Sat 12/02/22 | Barnoldswick Town v Runcorn Town | 1-2 | | | | | |
| Sat 19/02/22 | Skelmersdale United v Barnoldswick Town | 3-0 | | | | | |
| Sat 26/02/22 | Vauxhall Motors v Barnoldswick Town | 2-1 | | | | | |
| Sat 05/03/22 | Barnoldswick Town v Avro | 0-2 | | | | | |
| Sat 12/03/22 | Barnoldswick Town v Padiham | 2-0 | | | | | |
| Wed 23/03/22 | Barnoldswick Town v Wythenshawe Town | 1-1 | | | | | |
| Sat 26/03/22 | Ashton Athletic v Barnoldswick Town * | 2-2 | | | | | |
| Wed 30/03/22 | Barnoldswick Town v Charnock Richard | 0-3 | | | | | |
