| Sat 20/07/24 | Barnoldswick Town v Wythenshawe (CUP) | 1-2 | | | |  | | |
| Sat 27/07/24 | Barnoldswick Town v Lower Breck | 3-3 | | | | | | |
| Tue 30/07/24 | Colne v Barnoldswick Town | 1-4 | | | | | |  |
| Sat 03/08/24 | Barnoldswick Town v Brighouse Town (CUP) | 0-0 | |  | | | | |
| Tue 13/08/24 | Barnoldswick Town v Padiham | 2-2 | | | | | | |
| Sat 17/08/24 | Barnoldswick Town v Northwich Victoria (CUP) | 1-1 | | | | | | |
| Tue 20/08/24 | Northwich Victoria v Barnoldswick Town (CUP) | 2-4 | | | | | | |
| Sat 24/08/24 | Barnoldswick Town v Pilkington | 2-0 |  | | | | | |
| Mon 26/08/24 | Abbey Hey v Barnoldswick Town | 1-2 | | | | | | |
| Sat 31/08/24 | Lancaster City v Barnoldswick Town (CUP) | 0-0 | | | | | | |
| Tue 03/09/24 | Barnoldswick Town v Lancaster City (CUP) | 1-0 | | | | | | |
| Sat 07/09/24 | Barnoldswick Town v Cheadle Town | 1-0 | | | | | | |
| Tue 10/09/24 | Barnoldswick Town v Longridge Town | 2-1 | | | | | | |
| Sat 14/09/24 | Curzon Ashton v Barnoldswick Town (CUP) | 6-0 | | | | | | |
| Tue 17/09/24 | Irlam v Barnoldswick Town | 3-0 | | | | | | |
| Sat 21/09/24 | Barnoldswick Town v Steeton (CUP) | 3-1 | | | | | | |
| Tue 24/09/24 | Barnoldswick Town v Glossop North End | 0-1 | | | | | | |
| Sat 28/09/24 | Barnoldswick Town v FC Isle of Man | 1-3 | | | | | | |
| Tue 01/10/24 | Liversedge v Barnoldswick Town (CUP) | 1-2 | | | | | | |
| Sat 05/10/24 | West Didsbury & Chorlton v Barnoldswick Town | 2-1 | | | | | | |
| Tue 08/10/24 | Barnoldswick Town v Ramsbottom United | 1-1 | | | | | | |
| Sat 12/10/24 | Stockport Town v Barnoldswick Town | 0-1 | | | | | | |
| Sat 19/10/24 | Ryton & Crawcrook Albion v Barnoldswick Town (CUP) | 3-0 | | | | | | |
| Tue 22/10/24 | Barnoldswick Town v West Didsbury & Chorlton | 1-0 | | | | | | |
| Sat 26/10/24 | Barnoldswick Town v South Liverpool | 3-2 | | | | | | |
| Tue 29/10/24 | Squires Gate v Barnoldswick Town | 2-1 | | | | | | |
| Sat 02/11/24 | Prestwich Heys v Barnoldswick Town | 0-2 | | | | | | |
| Sat 09/11/24 | FC St Helens v Barnoldswick Town | 0-1 | | | | | | |
| Sat 16/11/24 | Barnoldswick Town v Irlam | 2-2 | | | | | | |
| Wed 20/11/24 | AFC Blackpool v Barnoldswick Town (CUP) | 4-1 | | | | | | |
| Wed 27/11/24 | Litherland REMYCA v Barnoldswick Town | 2-1 | | | | | | |
| Sat 30/11/24 | Longridge Town v Barnoldswick Town | 3-3 | | | | | | |
| Wed 11/12/24 | Cheadle Town v Barnoldswick Town | 1-3 | | | | | | |
| Sat 14/12/24 | Barnoldswick Town v AFC Liverpool | 1-1 | | | | | | |
| Sat 21/12/24 | Glossop North End v Barnoldswick Town | 3-0 | | | | | | |
| Thu 26/12/24 | Barnoldswick Town v Colne | 2-3 | | | | | | |
| Sat 04/01/25 | South Liverpool v Barnoldswick Town | 3-1 | | | | | | |
| Sat 18/01/25 | Barnoldswick Town v Bury | 1-4 | | | | | | |
| Tue 21/01/25 | Barnoldswick Town v Golcar United (CUP) | 2-3 | | | | | | |
| Sat 25/01/25 | Burscough v Barnoldswick Town | 2-1 | | | | | | |
| Sat 01/02/25 | Barnoldswick Town v Squires Gate | 4-1 | | | | | | |
| Tue 04/02/25 | Barnoldswick Town v Charnock Richard | 0-4 | | | | | | |
| Sat 08/02/25 | Barnoldswick Town v Litherland REMYCA | 2-1 | | | | | | |
| Tue 11/02/25 | Barnoldswick Town v FC St Helens | 4-1 | | | | | | |
| Sat 15/02/25 | FC Isle of Man v Barnoldswick Town | 1-1 | | | | | | |
| Tue 18/02/25 | Padiham v Barnoldswick Town | 0-2 | | | | | | |
| Sat 22/02/25 | Barnoldswick Town v Stockport Town | 1-1 | | | | | | |
| Sat 01/03/25 | Pilkington v Barnoldswick Town | 2-0 | | | | | | |
| Sat 08/03/25 | Bury v Barnoldswick Town | 2-0 | | | | | | |
| Sat 15/03/25 | Barnoldswick Town v Abbey Hey | 2-2 | | | | | | |
| Sat 22/03/25 | Chadderton v Barnoldswick Town | 1-1 | | | | | | |
| Fri 28/03/25 | AFC Liverpool v Barnoldswick Town | 2-0 | | | | | | |
| Sat 05/04/25 | Barnoldswick Town v Prestwich Heys | 2-3 | | | | | | |
| Sat 12/04/25 | Lower Breck v Barnoldswick Town | 6-0 | | | | | | |
| Sat 19/04/25 | Ramsbottom United v Barnoldswick Town | 1-1 | | | | | | |
