| Sat 09/08/25 | Litherland REMYCA v Barnoldswick Town * | 1-3 | | | | | | |
| Tue 12/08/25 | Barnoldswick Town v Chadderton * | 0-6 | | | |  | | |
| Sat 13/09/25 | West Didsbury & Chorlton v Barnoldswick Town * | 4-0 | | | | | | |
| Tue 16/09/25 | Longridge Town v Barnoldswick Town | 1-2 | | | | | | |
| Sat 20/09/25 | Barnoldswick Town v Sunderland West End (CUP) | 10-1 | | | | | | |
| Tue 23/09/25 | Barnoldswick Town v Stockport Town * | 3-3 | | | | | | |
| Sat 27/09/25 | Longridge Town v Barnoldswick Town (CUP) * | 1-3 | | | | | | |
| Sat 04/10/25 | FC Isle of Man v Barnoldswick Town | 1-2 | | | | | | |
| Sat 11/10/25 | AFC Blackpool v Barnoldswick Town (CUP) | 3-0 | | | | | | |
| Tue 14/10/25 | Barnoldswick Town v Ramsbottom United * | 1-3 | | | | | | |
| Sat 18/10/25 | Barnoldswick Town v AFC Liverpool (CUP) * | 1-0 | |  | | | | |
| Tue 21/10/25 | Irlam v Barnoldswick Town * | 1-2 | | | | | | |
| Sat 25/10/25 | Barnoldswick Town v Pilkington * | 6-1 | | | | | | |
| Tue 28/10/25 | Liversedge v Barnoldswick Town (CUP) | 2-2 | | | | | | |
| Sat 01/11/25 | Barnoldswick Town v Burscough * | 1-2 | | | | | | |
| Tue 04/11/25 | Ramsbottom United v Barnoldswick Town * | 4-1 | | | | | | |
| Sat 08/11/25 | Barnoldswick Town v Winsford United (CUP) | 1-2 | | | | | | |
| Tue 11/11/25 | Barnoldswick Town v Longridge Town | - | | | | | | |
| Sat 15/11/25 | Stockport Town v Barnoldswick Town * | 2-0 | | | | | | |
| Sat 22/11/25 | Barnoldswick Town v FC St Helens | 1-1 | | | | | | |
| Sat 29/11/25 | Barnoldswick Town v City of Liverpool | 7-0 | | | | | | |
| Sat 06/12/25 | Atherton LR v Barnoldswick Town * | 2-0 | | | | | | |
| Tue 16/12/25 | Club Thorne Colliery v Barnoldswick Town (CUP) | 0-3 | | | | | | |
| Sat 20/12/25 | Prestwich Heys v Barnoldswick Town * | 0-2 | | | | | | |
| Fri 26/12/25 | Padiham v Barnoldswick Town * | 3-1 | | | | | | |
