| Sat 02/08/25 | Darwen FC v MSB Woolton | 2-1 | | | | | | |
| Sat 09/08/25 | Maine Road v MSB Woolton | 5-0 | | | | | | |
| Sat 16/08/25 | MSB Woolton v Daisy Hill | 2-2 | | |  | | | |
| Sat 23/08/25 | Burscough v MSB Woolton (CUP) * | 4-2 | | | | | | |
| Wed 27/08/25 | MSB Woolton v Garstang (CUP) * | 5-1 |  | | | | | |
| Sat 30/08/25 | MSB Woolton v Holker Old Boys * | 0-0 | |  | |  | | |
| Sat 06/09/25 | Garstang v MSB Woolton | 2-2 | | | | | | |
| Sat 13/09/25 | Fulwood Amateurs v MSB Woolton * | 2-1 | | | | | | |
| Sat 20/09/25 | Ashton Athletic v MSB Woolton | 1-4 | | | | | | |
| Wed 24/09/25 | MSB Woolton v Bacup Borough | 4-3 | | | | | | |
| Sat 27/09/25 | Litherland REMYCA v MSB Woolton (CUP) | 2-2 | | | | | | |
| Tue 07/10/25 | Droylsden v MSB Woolton | 0-0 | | | | | | |
| Sat 11/10/25 | FC St Helens v MSB Woolton (CUP) | 0-2 | | | | | | |
| Sat 18/10/25 | Bacup Borough v MSB Woolton | 4-0 | | | | | | |
| Sat 25/10/25 | AFC Blackpool v MSB Woolton | 2-1 | | | | | | |
| Sat 01/11/25 | MSB Woolton v Colne | 3-3 | | | | | | |
| Sat 08/11/25 | Squires Gate v MSB Woolton | 1-2 | | | | | | |
| Wed 12/11/25 | Nelson v MSB Woolton (CUP) | 2-1 | | | | | | |
| Wed 26/11/25 | MSB Woolton v Steeton | 0-1 | | | | | | |
| Wed 03/12/25 | MSB Woolton v Bootle (CUP) * | 1-1 | | | | | | |
| Sat 06/12/25 | MSB Woolton v Garstang | 3-1 | | | | | | |
| Wed 10/12/25 | MSB Woolton v Nelson | 1-1 | | | | | | |
| Sat 13/12/25 | MSB Woolton v Ashton Athletic | 2-0 | | | | | |  |
| Sat 20/12/25 | Steeton v MSB Woolton | 0-1 | | | | | | |
| Sat 27/12/25 | MSB Woolton v Maghull | 4-2 | | | | | | |
| Sat 10/01/26 | MSB Woolton v Thornton Cleveleys | 2-1 | | | | | | |
| Tue 13/01/26 | MSB Woolton v Runcorn Linnets (CUP) | 1-2 | | | | | | |
| Sat 17/01/26 | Holker Old Boys v MSB Woolton | 2-2 | | | | | | |
