| Sat 26/07/25 | Darwen FC v Thornton Cleveleys | 0-0 | |  | | | | |
| Sat 02/08/25 | Darwen FC v MSB Woolton * | 2-1 | | | | | | |
| Sat 09/08/25 | Squires Gate v Darwen FC * | 2-1 | | | |  | | |
| Sat 30/08/25 | Darwen FC v AFC Blackpool | 0-2 | | | | | | |
| Sat 20/09/25 | Armthorpe Welfare v Darwen FC (CUP) * | 2-6 | | | | | | |
| Sat 04/10/25 | Darwen FC v Maghull * | 5-2 | | | | | | |
| Sat 11/10/25 | Daisy Hill v Darwen FC | 3-2 | | | | | | |
| Sat 18/10/25 | Ashton Town v Darwen FC (CUP) * | 1-0 | | | | | | |
| Sat 25/10/25 | Darwen FC v Garstang * | 4-0 | | | | | | |
| Wed 29/10/25 | Darwen FC v Colne (CUP) * | 4-0 |  | | | | | |
| Sat 01/11/25 | Darwen FC v Ashton Athletic * | 3-1 | | | | | | |
| Sat 08/11/25 | Colne v Darwen FC * | 4-1 | | | | | | |
| Sat 15/11/25 | Ashton Town v Darwen FC (CUP) * | 2-3 | | | | | | |
| Wed 19/11/25 | Darwen FC v Bacup Borough * | 1-1 | | | | | | |
| Sat 22/11/25 | Maghull v Darwen FC * | 1-1 | | | | | | |
| Sat 29/11/25 | Darwen FC v Ashton Town * | 1-0 | | | | | | |
| Sat 06/12/25 | Ashton Athletic v Darwen FC * | 2-2 | | | | | | |
| Sat 13/12/25 | Darwen FC v Holker Old Boys * | 3-3 | | | | | | |
| Sat 10/01/26 | Darwen FC v Droylsden * | 2-3 | | | | | | |
| Sat 17/01/26 | Ashton Town v Darwen FC * | 2-0 | | | | | | |
| Tue 20/01/26 | Fulwood Amateurs v Darwen FC * | 2-2 | | | | | | |
| Sat 07/02/26 | Thornton Cleveleys v Darwen FC * | 3-7 | | | | | | |
| Sat 07/03/26 | Garstang v Darwen FC * | 2-1 | | | | | | |
| Sat 14/03/26 | Darwen FC v Stockport Georgians (CUP) * | 1-0 | | | | | | |
| Wed 25/03/26 | Darwen FC v Steeton * | 2-0 | | | | | | |
| Sat 28/03/26 | MSB Woolton v Darwen FC * | 2-1 | | | | | | |
| Sat 18/04/26 | Droylsden v Darwen FC | 2-2 | | | | | | |
| Thu 30/04/26 | Darwen FC v Runcorn Town (CUP) * | 2-3 | | | | | | |
