| Sat 27/07/24 | Holker Old Boys v Daisy Hill | 1-1 | | | | | | |
| Wed 31/07/24 | Garstang v Holker Old Boys * | 1-3 | | | | | | |
| Sat 03/08/24 | Holker Old Boys v Droylsden | 4-0 | |  | | | |  |
| Tue 06/08/24 | Holker Old Boys v Euxton Villa | 2-2 | | | | | | |
| Sat 10/08/24 | Ashton Town v Holker Old Boys | 0-3 |  | | | | | |
| Mon 12/08/24 | Route One Rovers v Holker Old Boys | 1-3 | | | | | | |
| Sat 17/08/24 | Holker Old Boys v Nelson | 4-1 | | | | | | |
| Wed 21/08/24 | AFC Blackpool v Holker Old Boys | 5-1 | | | | | | |
| Sat 24/08/24 | Daisy Hill v Holker Old Boys | 0-1 | | | | | | |
| Mon 26/08/24 | Holker Old Boys v Maghull | 3-0 | | | | | | |
| Sat 31/08/24 | Holker Old Boys v Bacup Borough | 3-1 | | | | | | |
| Sat 07/09/24 | Darwen FC v Holker Old Boys | 2-4 | | | | | | |
| Sat 14/09/24 | Steeton v Holker Old Boys | 2-5 | | | | | | |
| Sat 21/09/24 | Crook Town v Holker Old Boys (CUP) | 0-0 | | | | | | |
| Sat 28/09/24 | Holker Old Boys v Maine Road | 3-2 | | | | | | |
| Tue 01/10/24 | Longridge Town v Holker Old Boys (CUP) | 1-1 | | | | | | |
| Sat 05/10/24 | Skelmersdale United v Holker Old Boys | 0-9 | | | | | | |
| Tue 08/10/24 | Holker Old Boys v Darwen FC (CUP) | 1-0 | | | | | | |
| Sat 26/10/24 | Holker Old Boys v AFC Blackpool * | 2-4 | | | | | | |
| Sat 02/11/24 | Atherton LR v Holker Old Boys * | 2-0 | | | | | | |
| Sat 09/11/24 | Daisy Hill v Holker Old Boys (CUP) | 1-2 | | | | | | |
| Sat 16/11/24 | Bacup Borough v Holker Old Boys | 1-1 | | | | | | |
| Sat 14/12/24 | Holker Old Boys v Skelmersdale United | 4-0 | | | | | | |
| Thu 26/12/24 | Thornton Cleveleys v Holker Old Boys | 1-3 | | | | | | |
| Sat 04/01/25 | Euxton Villa v Holker Old Boys | 3-2 | | | | | | |
| Sat 18/01/25 | Holker Old Boys v Ashton Athletic | 3-2 | | | | | | |
| Sat 01/02/25 | Abbey Hulton United v Holker Old Boys (CUP) | 1-3 | | | | | | |
| Sat 08/02/25 | Holker Old Boys v Darwen FC | 1-1 | | | | | | |
| Sat 15/02/25 | Holker Old Boys v Steeton | 2-0 | | | | | | |
| Sat 22/02/25 | Ashton Athletic v Holker Old Boys | 1-0 | | | | | | |
| Sat 01/03/25 | Maghull v Holker Old Boys | 1-1 | | | | | | |
| Sat 08/03/25 | Holker Old Boys v Atherton LR | 3-1 | | | | | | |
| Sat 15/03/25 | Holker Old Boys v Atherton LR (CUP) | 1-2 | | | | | | |
| Mon 17/03/25 | Maine Road v Holker Old Boys | 0-0 | | | | | | |
| Sat 22/03/25 | Holker Old Boys v Ashton Town | 2-2 | | | | | | |
