| Sat 27/07/24 | Route One Rovers v Garstang | 0-2 | | | | | | |
| Mon 29/07/24 | Route One Rovers v Bacup Borough | 0-2 | | | | | | |
| Sat 03/08/24 | Thornton Cleveleys v Route One Rovers | 4-1 | | | | | | |
| Tue 06/08/24 | Steeton v Route One Rovers | 1-2 | | | | | | |
| Sat 10/08/24 | Route One Rovers v Ashton Athletic | 0-1 | | | |  | | |
| Sat 07/09/24 | Daisy Hill v Route One Rovers | 1-0 | | | | | | |
| Sat 14/09/24 | Droylsden v Route One Rovers | 0-0 | |  | | | | |
| Sat 28/09/24 | Route One Rovers v Darwen FC | 3-2 | | | | | | |
| Mon 30/09/24 | Route One Rovers v AFC Blackpool (CUP) | 1-3 |  | | | | | |
| Sat 05/10/24 | Route One Rovers v Atherton LR | 0-3 | | | | | | |
| Mon 07/10/24 | Route One Rovers v Ashton Athletic (CUP) | 3-0 | | | | | | |
| Sat 12/10/24 | Nelson v Route One Rovers | 5-1 | | | | | | |
| Tue 15/10/24 | Harrogate Railway Athletic v Route One Rovers (CUP) | 1-3 | | | | | | |
| Sat 26/10/24 | Maine Road v Route One Rovers | 3-1 | | | | | | |
| Sat 02/11/24 | Maghull v Route One Rovers | 3-5 | | | | | | |
| Sat 16/11/24 | Route One Rovers v AFC Blackpool | 2-3 | | | | | | |
| Mon 18/11/24 | Route One Rovers v Armthorpe Welfare (CUP) | 6-3 | | | | | | |
| Sat 30/11/24 | Holker Old Boys v Route One Rovers | 0-2 | | | | | |  |
| Mon 02/12/24 | Route One Rovers v Atherton LR (CUP) | 1-2 | | | | | | |
| Sat 14/12/24 | Bacup Borough v Route One Rovers | 2-2 | | | | | | |
| Thu 26/12/24 | Route One Rovers v Steeton | 0-2 | | | | | | |
| Sat 28/12/24 | Route One Rovers v Nelson | 0-4 | | | | | | |
| Sat 18/01/25 | Darwen FC v Route One Rovers | 4-0 | | | | | | |
| Sat 25/01/25 | Route One Rovers v Skelmersdale United | 2-0 | | | | | | |
| Mon 03/02/25 | Albion Sports v Route One Rovers (CUP) | 1-3 | | | | | | |
| Sat 08/02/25 | Route One Rovers v Daisy Hill | 2-3 | | | | | | |
| Sat 15/02/25 | Route One Rovers v Droylsden | 1-3 | | | | | | |
| Sat 22/02/25 | Route One Rovers v Euxton Villa | 1-4 | | | | | | |
| Sat 01/03/25 | Ashton Town v Route One Rovers | 1-2 | | | | | | |
| Sat 22/03/25 | Garstang v Route One Rovers | 3-2 | | | | | | |
| Sat 29/03/25 | Route One Rovers v Maghull | 1-3 | | | | | | |
| Tue 01/04/25 | Euxton Villa v Route One Rovers | 1-3 | | | | | | |
| Sat 05/04/25 | Ashton Athletic v Route One Rovers | 1-4 | | | | | | |
| Wed 09/04/25 | Campion v Route One Rovers (CUP) | 1-2 | | | | | | |
| Sat 12/04/25 | Route One Rovers v Thornton Cleveleys * | 1-3 | | | | | | |
| Tue 22/04/25 | Golcar United v Route One Rovers (CUP) | 2-2 | | | | | | |
