| Sat 31/07/21 | Runcorn Town v Barnoldswick Town | 1-2 | | |  | | |
| Tue 03/08/21 | Barnoldswick Town v Squires Gate | 2-2 |  | | | | |
| Sat 07/08/21 | Barnoldswick Town v Runcorn Town | 6-1 | | | | | |
| Sat 14/08/21 | Barnoldswick Town v Prestwich Heys | 3-1 | | | | | |
| Wed 18/08/21 | Longridge Town v Barnoldswick Town | 2-5 | | | | | |
| Sat 21/08/21 | Barnoldswick Town v Ramsbottom United | 5-4 | | | | | |
| Sat 28/08/21 | Barnoldswick Town v Macclesfield | 1-2 | | | | | |
| Mon 30/08/21 | Padiham v Barnoldswick Town | 0-0 | | | | |  |
| Sat 04/09/21 | Barnoldswick Town v Pontefract Collieries | 0-3 | | | | | |
| Tue 07/09/21 | Burscough v Barnoldswick Town | 2-0 | | | | | |
| Sat 11/09/21 | Barnoldswick Town v AFC Blackpool | 3-3 | | | | | |
| Sat 18/09/21 | Barnoldswick Town v Vauxhall Motors | 2-3 | | | | | |
| Tue 21/09/21 | Irlam v Barnoldswick Town | 0-3 | | | | | |
| Sat 19/02/22 | Skelmersdale United v Barnoldswick Town * | 3-0 | | | | | |
| Sat 26/02/22 | Vauxhall Motors v Barnoldswick Town * | 2-1 | | | | | |
| Sat 05/03/22 | Barnoldswick Town v Avro | 0-2 | | | | | |
| Sat 12/03/22 | Barnoldswick Town v Padiham | 2-0 | | | | | |
| Tue 15/03/22 | Prestwich Heys v Barnoldswick Town | 0-2 | | | | | |
| Sat 19/03/22 | Barnoldswick Town v Lower Breck | 1-0 | | | | | |
| Wed 23/03/22 | Barnoldswick Town v Wythenshawe Town | 1-1 | | | | | |
| Sat 26/03/22 | Ashton Athletic v Barnoldswick Town * | 2-2 | | | | | |
| Wed 30/03/22 | Barnoldswick Town v Charnock Richard | 0-3 | | | | | |
| Sat 02/04/22 | Northwich Victoria v Barnoldswick Town | 2-1 | | | | | |
| Tue 12/04/22 | Bury AFC v Barnoldswick Town | 3-1 | | | | | |
