| Tuesday 30th September 2025 | The Macron Cup 1st Rnd | ||
| 19:45 |  Irlam v Chadderton  | The Silver St Stadium | |
| Saturday 11th October 2025 | The Macron Cup 2nd Rnd | ||
| 15:00 |  AFC Blackpool v Barnoldswick Town  | Mechanics Ground | |
| 15:00 |  AFC Liverpool v Maghull  | Berry Street Garage Stadium | |
| 15:00 |  Atherton LR v Burscough  | Crilly Park | |
| 15:00 |  Cammell Laird 1907 v Wolverhampton Casuals  | The OWS Engineering Ltd Stadium | |
| 15:00 |  City of Liverpool v Charnock Richard  | The Community Ground | |
| 15:00 |  Colne v Euxton Villa  | Haffners Eats Stadium | |
| 15:00 |  Eccleshall v Telford Town  | Stoke Signage Stadium | |
| 15:00 |  FC St Helens v MSB Woolton  | SMS PRO SOCCER PARK | |
| 15:00 |  Foley Meir v Sandbach United  | Trentham Foods Stadium | |
| 15:00 |  Garstang v Thornton Cleveleys  | The Riverside | |
| 15:00 |  Maine Road v Cheadle Town  | Brantingham Road | |
| 15:00 |  Padiham v Nelson  | Ruby Civil Arena | |
| 15:00 |  Ramsbottom United v Prestwich Heys  | Harry Williams Riverside Stadium | |
| 15:00 |  Runcorn Town v Allscott Heath  | Viridor Community Stadium | |
| 15:00 |  West Didsbury & Chorlton v Abbey Hey  | Step Places Stadium | |
| Tuesday 30th September 2025 The Macron Cup 1st Round |
| Irlam v Chadderton (19:45) |
| Saturday 11th October 2025 The Macron Cup 2nd Round |
| AFC Blackpool v Barnoldswick Town (15:00) |
| AFC Liverpool v Maghull (15:00) |
| Atherton LR v Burscough (15:00) |
| Cammell Laird 1907 v Wolverhampton Casuals (15:00) |
| City of Liverpool v Charnock Richard (15:00) |
| Colne v Euxton Villa (15:00) |
| Eccleshall v Telford Town (15:00) |
| FC St Helens v MSB Woolton (15:00) |
| Foley Meir v Sandbach United (15:00) |
| Garstang v Thornton Cleveleys (15:00) |
| Maine Road v Cheadle Town (15:00) |
| Padiham v Nelson (15:00) |
| Ramsbottom United v Prestwich Heys (15:00) |
| Runcorn Town v Allscott Heath (15:00) |
| West Didsbury & Chorlton v Abbey Hey (15:00) |
